Isolation of Tightly Coupled Mitochondria From Acidic Plant Tissues
نویسندگان
چکیده
منابع مشابه
Isolation of tightly coupled mitochondria from acidic plant tissues.
T'b is comilitmication emphasizes the nieed for conltin1uouts conitrol of pH when macerating acidic plant tissues for the l)url)ose of isolating tightly coupled mitochondria. A useful device for such control is described. Mitochonidria capable of oxidative but not pl)osphorylative activity have been isolated fromli apple fruits wlhenl either alkaline homogeniates (5) or l)oIyvinylpyrrolidone (P...
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A number of parameters associated with the isolation of plant mitochondria were carefully examined in the hope of optimizing the isolation technique. Dark-grown mung bean (Phaseolus aureus var. Jumbo) hypocotyls were used as the source of mitochondria. Necessary conditions for the isolation of tightly coupled plant mitochondria include: (a) gentle tissue disruption; (b) rigorous exclusion of co...
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The mitochondria isolated from dark-grown mung bean hypocotyls oxidize succinate, l-malate, and externally added reduced nicotine adenine dinucleotide (NADH) with good respiratory control. While the pattern of respiration resembles that of animal mitochondria, there are 4 basic differences between the respiratory properties of mung bean and animal mitochondria: A) the ability to oxidize NADH, B...
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Mitochondria are complex organelles at the center of cellular metabolism, apoptosis, and signaling. They continue to be the subject of intense basic investigation to understand their composition and function, but they have also captivated the attention of clinical researchers because of the growing knowledge of the (sometimes unexpected) roles of mitochondria in human diseases and aging. A full...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1969
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.44.2.311